Journal: Discover Oncology
Article Title: Lidocaine suppresses HER2-positive breast cancer cell proliferation by targeting the OGT-CCNL1 axis
doi: 10.1007/s12672-025-04194-4
Figure Lengend Snippet: Effects of lidocaine on the proliferation and cell cycle distribution of HER2-positive breast cancer cells. A Chemical structure of lidocaine. B CCK-8 assay showing dose-dependent reduction in cell viability of HER2-positive breast cancer cells (AU565 and BT474 cells) and non-tumorigenic immortalized mammary epithelial MCF-10 A cells after lidocaine treatment (0.5, 1, and 2 mM). C EdU incorporation assay illustrating the suppression of DNA synthesis and proliferative activity in lidocaine-treated HER2-positive breast cancer cells. D Flow cytometry analysis of cell cycle distribution in AU565 and BT474 cells treated with lidocaine at 0.5, 1 and 2 mM concentrations. Data are presented as mean ± SD ( n = 3), one - way ANOVA combined with Tukey’s post - hoc test was used for comparison among multiple groups
Article Snippet: The human HER2-positive breast cancer cell lines (AU565 and BT474) and non-tumorigenic epithelial cell line MCF-10 A were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA).
Techniques: CCK-8 Assay, DNA Synthesis, Activity Assay, Flow Cytometry, Comparison